MORPHOLOGICAL CHANGES IN LOACH EMBRYO MISGURNUS FOSSILIS L. UNDER THE INFLUENCE OF FTORHINOLONS CLASS OF ANTIBIOTICS

In the article shown morphological changes in embryos of loach Misgurnus fossilis L. under the action of antibiotic class fluoroquinolone, borocine and flumiqvil, in different concentrations of 0.1%, 0.01%, 0.05% and 0.005% during embryogenesis. Shown that flumiqvil inhibition of embryos viability was more then borotsyn at drug concentrations of 0.01%, 0.05% and 0.005%. Abnormalities were not observed at the initial stages of embryos, although the division into two blastomeres occurred later than in control (1.5-2 hours after fertilization) under the action of flumiqvil. During the next stages of division, the blastomerization was normalized. Due to some specific features of the blastomeres of loach liver, they cannot be equated to the division of somatic cells. Because in the early stages of embryogenesis of loach there is no synthesis of some components of cell mass. Preparation of cell division here characterized by the synthesis of mainly DNA, RNA, histones, proteins of the mitochondria, compounds rich in energy. Consequently, in the period of synchronous blastomorphism, a number of physicochemical processes are involved, which are related to the formation of trigger and regulatory mechanisms of mitosis. Since many toxic substances inhibit these cellular processes, it attracts the attention of researchers to the use of loach embryos as an object in a toxicological experiment. Dysregulation of the molecular organization of membranes or the destruction of antioxidants, as well as a number of other poorly studied factors, which occur under the influence of toxic substances, can lead to increased reactions of peroxidation and the formation of many pathological processes in the cell. Received results suggest that the embryos of loach Misgurnus fossilis L. at the stage of early embryogenesis is a convenient and adequate test-system for studying the influence of various pharmacological, chemical and biologically active factors on living objects.