Biosynthesis of Pyramidical Nanosilver Using Andrographis paniculata (Burm.f.) and its Efficacy against Anopheles stephensi (Liston) Larvae in Laboratory

We have tested the larvicidal efficacy of AgNPs (Silver nanoparticles) synthesized by using stem extract of Andrographis paniculata (Burm.f.). The AgNPs synthesized using green bio-reduction methods which is economical as well as ecofriendly. The AgNPs formation was confirmed by UV-VIS spectrophotometer, XRD (X-Ray Diffraction) and SEM (Scanning Electron Microscopy). It reveals the geometry including size, shape and morphology of synthesized AgNPs. The biosynthesized AgNPs were spherical, L shaped and Pyramidical shaped, also the average sizes of synthesized silver nanoparticles were found to be 9.59 nm. The larvicidal bioassay further reveals LC50 and LC90 values against I, II and III instars of Anopheles stephensi (Liston) after 24 hr and 48 hr The AgNPs synthesized using stem extract of Andrographis paniculata have potent effect on larvae of Anopheles stephensi. Silver nanoparticles synthesized by stem extract of Andrographis paniculata are effective and economical with its eco-friendly characteristics. For controlling Anopheles stephensi in its larval stage this can be useful in tropical countries having malarial dominance. Further the Pyramidical structure observed in our study can be used in drug delivery for disease like malaria, cancer, dengue, etc