Etiology and antimicrobial resistance patterns of Extended Spectrum Beta Lactamases (ESBL) producing E. coli from urinary tract infections in Tehsil Head Quarter (THQ) Hospital, Burewala, Pakistan

Objectives: The present study was conducted to isolate Extended Spectrum Beta-Lactamases(ESBL) producing strain of E. coli causing UTIs in hospitalized patients and to evaluate antimicrobial resistance pattern of most commonly used antibiotics in treatment therapy, in Tehsil headquarter hospital in Burewala. Methods: A total of 150 samples were collected from Patients diagnosed with urinary tract infections (UTIs), from Tehsil Head Quarter (THQ) Hospital, Burewala, Pakistan. All samples were streaked on specific media, MacConkey agar where positive isolates of E. coli showed pink colored colonies, further confirmation was done by performing some biochemical tests. ESBL detection of E. coli isolates was confirmed by performing Double Disc Synergy Test (DDST). In the end antimicrobial resistance pattern was checked by using some commonly used antibiotics such as ampicillin, amoxi-clavulanic acid combination, ciprofloxacin, norfloxacin, TMP-SMX, ceftriaxone and nitrofurantoin and results were observed. Results: A total of positive 58 samples for E. coli were detected and out of 58 samples, 43 samples were positive for ESBL-E. coli strain and rests were non-ESBL producers. The results showed higher prevalence of positive ESBL producer strain of E. coli in urinary tract infections and this in turn indicates higher positive ratio of 74.1% in UTIs among patients admitted in Tehsil Head Quarter (THQ) Hospital, Burewala. Conclusion: The outcome of our study was that higher prevalence of ESBL-E. coli (74.1%) was present among parents having UTIs, and maximum resistance was observed against ampicillin (89%) and ciprofloxacin (83.7%), however minimum resistance was observed against ceftriaxone (16%) and nitrofurantoin (14%), means that these drugs are effective in treatment therapy for UTIs, among patients in THQ, Burewala, Pakistan.