COMPOSITION OF PERIPHERAL BLOOD LEUKOCYTES: IMMUNE SYSTEM OF LYM-PHOCYTES IN WOMEN WITH OBESITY, DIA-BETES MELLITUS TYPE 2 PROVIDED WITH INSULIN-DEPENDENT AND NON- INSULIN-DEPENDENT THERAPY

Meager and ambivalent results about the role of the immune system in the pathogenesis of diabetes mellitus type II with obesity. There is a view that performances of some indicators of natural and adaptive immunity in patients with diabetes mellitus type II with obesity are due not only to diabetes, but also to the combination of obesity. It has now been discovered that adipose tissue is a powerful secretory organ that produces adipokines (leptin, adiponectin, etc.) They play a key role in various immune responses. However, it is known that the level of major adipokines regulating adipose tissue in peripheral blood of a person is determined taking into account sexual dimorphism, higher concentration among women than among men. Therefore, objective in the work was to know how change white blood cell count and immune properties of blood lymphocytes in insulin-dependent and non-insulin-dependent obese women with diabetes mellitus type II. Materials and methods of research. The 30 women with diabetes mellitus type II aged between 30 and 50 years were examined, which were divided into two groups: the first – with normal body weight (body mass index (BMI) < 25 kg / m2) and with obesity (BMI > 30 kg / m2). In addition, obese women have been di-vided into two subgroups of patients who are insulin-dependent and non-insulin-dependent. Consequently, the control group consisted of 30 relatively healthy women, same age, who were also divided into two groups: with normal BMI (<25 kg / m2) and with BMI > 29.9 kg / m2. Obesity was diagnosed by determining BMI. Total num-ber of leukocytes was counted using hematological ana-lyzer in the peripheral blood. Leucogram was studied in smears of peripheral blood for 200 cells (Romanowsky-Giemsa stain). The lymphocyte content was determined by laser flow cytometry system. Mononuclear cells were isolated from heparinized blood by differential centrifu-gation. Results of the research and their discussion. We have found statistically significant leukocytosis in all groups through monocytosis and neutrophilosis in obese women. According to our data, insulin-dependent women had a higher immune response by increasing lympho-cytes. By using insulin, the number of neutrophils and monocytes decreased, said that there had been a decrease in the inflammatory response of the body to insulin-dependent patients. Conclusions. There are more severe violations of the leukocyte composition peripheral blood and lym-phocytic immunity in women with diabetes mellitus type II and obesity compared with women without obesity. That explains the presence of more pronounced chronic inflammation and the reason for the more severe course of diabetes mellitus type II and obesity. However, the use of insulin therapy reduces the inflammatory of combined diseases (diabetes mellitus type II and obesity). Data suggest, however, that the effectiveness of using insulin causes a higher anti-inflammatory effect than the use of oral hypoglycemic drugs.