Bacteriological profile in UTI Cases with special reference to Amikacin sensitivity in a tertiary Care centre in Western UP

Introduction: UTI is the most common bacterial infection worldwide with adult women being 30 times more likely to suffer from it than men due to short urethra and proximity of uretheral opening with anal canal. Escherichia coli is the most common organism responsible for UTI and there has been an increase in incidence of community acquired UTI caused by ESBL producing E. coli. This study was planned to see the sensitivity of amikacin in urinary isolates so it can be considered as a treatment option in ESBL producing organisms, reserving high-end drugs like carbapenems. Materials and Methods: The retrospective study was carried out between January 2017 to August 2017 with 1025 patients of suspected UTI being screened for growth of bacteria in their urine samples and their sensitivity to antibiotics. Both males and females were included in the study, ages ranging from 0 year to >80 yrs. Urine samples were inoculated on Blood agar and MacConkey agar. All positive cultures were tested for antibiotic susceptibility testing by Kirby bauer disc diffusion technique. Results: Out of total 1025 samples, growth was detected in 171 (16.7%) samples. Out of these 121 (70.8%) were females, and 50 (29.2%) were males. Maximum number of males are seen in age group 21-30 yrs i.e. 13 (26%) followed by 41 to 50 yrs i.e. 12 (24%). Also, maximum number of females are seen in age group 21-30 yrs i.e. 41 (33.9%) followed by 21 (17.4%) patients in age group 41-50yrs. Most common microbial agent isolated was E.coli (97/171), followed by Klebsiella spp.(25/171). The important finding of the study was the sensitivity pattern of amikacin in this area which was found to be sensitive in 61% of the isolates which is quite significant as we can use amikacin rather than high end antibiotics like carbapenems in sensitive cases in UTI and prevent emergence of resistance against them. Conclusion: Our finding i.e. Amikacin being highly sensitive to urinary pathogens reflects upon the facts that in sensitive cases, Amikacin can be used for ESBL producing uropathogens instead of costly high end antibiotics like Carbapenams.