In vitro propagation and Abrobacterium mediated transformation studies on chenopodium album

Tissue explants from different parts of an important Indian medicinal Plant, Chenopodium album L, were cultured in vitro and their morphogenetic potential was elucidated in the present investigation. Explants from leaf, stem and node were cultured on MS medium supplemented with different concentration and combination of plant hormones like IAA, NAA, 2, 4-D and BAP. It was observed that stem explants have maximum morphogenetic potential than other explants of chenopodium album. Optimum concentration of individual hormones of the growth of the stem explant was determined. It was found to be 2 mg/l for IAA and NAA, 0.5 mg/l for 2, 4-D and 0.5 mg/l for BAP. Factorial combination of different concentration and combination of plant hormones was tried to ellicit morphogenetic potential of stem explants, compact, green colored and non-friable callus was formed on medium supplemented with IAA of NAA, white friable callus was formed on medium supplemented with 2, 4-D. For maximum callus for-mation on stem explants a medium supplemented with 2, 4-D 0.5 mg/l and BAP 1 mg/l was found to be ideal callus could be sub cultured indefinatly on this medium for several passages. Multiple shoots were formed on stem explant derived callus on medium supplemented with IAA 1 mg/l and BAP 2 mg/l. Two or three shoots per explant was obtained. The amount of phenol present in the callus extracts on different hormones ....