Effect of mutation on antomictobial activity of actinomycetes from western ghat’s of India

A total of 56 Actinomycetes strains were isolated from Indian western ghat’s soil and their antagonistic activities were tested against six human pathogenic bacteria viz, Escherichia coli, Staphylococcus aureus, Salmonella typhi, Shigella sp.,Klebsiella pneumonia and Bacillus subtilis. Out of 56 strains isolated, only ten strains (WGA2, WGA7, WGA12, WGA15, WGA19, WGA21, WG29, WGA31, WGA37, and WGA49) showed high antibacterial activity against all the tested human bacterial pathogens. Among these, only 5 effective antagonistic strains (WGA7, WGA15, WGA21, WGA37 and WGA49) were selected for mutation study by using physical (UV radiation) and chemical (NTG) mutation methods. After the mutations, antibacterial activity of the strains viz WGA15, WGA49 (UV treated) WGA37 (NTG treated) was increased, but the WGA37 UV treated strain showed a decreased antibacterial activity. All the five strains possess LL-DAP and all the strains contained glycine in their cell wall. Presence of LLdiaminopimelicacid indicates that the cell wall having chemo type?1. So the cell wall property of the genes was identified the Streptomycetes.