Simultaneous Estimation of Cefixime and Cloxacillin in Bulk and Tablet Formulation by Rp-Hplc Method

A simple, economic, selective, precise and accurate high-performance liquid chromatographic (RP-HPLC) method for the analysis of cefixime and cloxacillin in both bulk drug and pharmaceutical formulations was developed and validated in the present study. This method has shown good resolution for cefixime, cloxacillin and formulation excipients of tablets. The mobile phase consisted of phosphate buffer (25 mM):methanol: acetonitrile (40: 45: 15 v/v/v), pH 4.6 adjusted with orthophosphoric acid. HPLC analysis of cefixime and cloxacillin was carried out at a wavelength of 225 nm with flow rate of 1.0 mL min-1. The method was quantitatively evaluated in terms of linearity, precision, accuracy (recovery), selectivity and robustness. The method is simple, convenient and suitable for the analysis of cefixime and cloxacillin in bulk and its formulation.