ASSESSMENT OF HEMOSTATIC ACTIVITY OF LOCAL HEMOSTATIC APPLICATION IMPLANTS BASED ON CELLULOSE DERIVATIVES IN IN VITRO EXPERIMENTS

Nowadays injuries of internal organs are rather common, they are accompanied by bleeding and require use of local intraoperative hemostatic measures including chemical means – local application spongy implants. Local hemostatic agents based on polymeric materials: sodium alginate, chitosan, cellulose, gelatin and so forth, are widely used by surgeons. Aim. Was assessment of hemostatic activity of local spongy hemostatic application implants based on sodium-carboxymethyl cellulose in in vitro experiment. Materials and Methods. Were new samples of hemostatic sponges based on sodium-carboxymethyl cellulose with technological modifications such as chemical recharge, various softeners and addition of medicines, developed jointly with OOO Linteks, Saint-Petersburg. The research object was native blood of 10 healthy volunteer donors. In an experiment changes in blood coagulation time were evaluated, mass and volume of the blood clot were determined. Results. It may be noted that the aminoacetic and salicylic acids used in samples №6 and №7 to the most extent enhanced hemostatic activity of polymeric sponges in comparison with other studied samples. Samples №2 and №3 showed the minimal positive result that permits to make a conclusion about low hemostatic properties of dextrin (sample №3) and also of partial chemical ‘recharge' (sample №2). Conclusion. All experimental samples showed statistically significant reduction of the time of blood clot formation, of the volume and mass of a clot in comparison with normal coagulation parameters. Samples № 6 and № 7 reduced blood clotting time in comparison with sample №1. Samples №2-7 showed higher clot volume parameters as compared to sample № 1. Samples №2 and №5 showed reduction of the mass of a clot as compared to control, and samples 3, 4, 6 and 7 showed increase in this parameter.