PARTIAL CHARACTERISATION AND THERAPEUTIC APPLICATION OF PROTEASE FROM A FUNGAL SPECIES
Journal: Indian Drugs (Vol.49, No. 10)Publication Date: 2012-10-28
Authors : Kalaskar V. V. Narayanan K. Subrahmanyam V. M.; Rao V. J.;
Page : 42-46
Keywords : *E-mail: vm.subra@manipal.edu;
Abstract
Proteases find wide application in textile, leather, food and pharmaceutical industries. Also this enzyme has considerable therapeutic importance in treating inflammation, digestive and vascular conditions. A protease obtained from a fungal isolate, Aspergillus niger, was tested for clinical application. The microorganism was cultivated on a starch-casein medium. The proteolytic activity was assayed with casein substrate using Folin-Ciocalteus method having tyrosine standard. The enzyme was precipitated using 50% ammonium sulphate. Inhibition studies on EDTA, phenyl methyl sulphonyl fluoride, dithiothreitol and pepstatin A revealed that the enzyme is an acid protease. The precipitated protease exhibited significant dehairing, destaining, declotting activites on Wistar rat model. The enzyme showed prominent anti-inflammatory activity and results were compared using diclofenac sodium.
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