SYNTHESIS OF NOVEL BENZOTRIAZOLYL FORMAZANS AND THEIR ANTIMICROBIAL AND ANTIOXIDANT ACTIVITY

Benzotriazolyl hydrazide (2) has been obtained from the ester ethyl benzotriazolyl acetate (1). Thehydrazide with various aromatic aldehydes in presence of glacial acetic acid gave correspondingarylidenes 3(a-h). The arylidenes were made to react with fluoro substituted diazonium salt whereby,benzotriazolyl formazans 4(a-h) were obtained. The structures of the compounds were confirmed by FTIR,1H-NMR, mass spectral data and elemental analysis. All the synthesized compounds were screenedfor their in-vitro antimicrobial activity against the bacterial strains Bacillus subtilis and Escherichia coli andthe fungal strains Asperigillus niger and Candida albicans by two fold serial dilution technique and alsofor their antioxidant activity. Among the compounds tested, compound 4b (fluoro substituted derivative)showed MIC as low as 15.6 ?g/mL against Bacillus subtilis and Candida albicans. Other derivatives alsoshowed moderate antimicrobial activity. Also, compound 4e (methoxy substituted derivative) showedsignificant antioxidant activity when compared to the standard, ascorbic acid.