The Effects of Quercetin to Glutathione System in the Bacterial-Immune Periodontitis Development

Perfecting the known and elaboration of new methods of periodontitis treatment is one of the important tasks and requires extraordinary approaches to their solutions. The purpose of the study was to determine effectiveness of water-soluble liposomal form of quercetin in correction of the glutathione system disorders in the experimental periodontitis development. The article presents the results of glutathione system researches in antioxidant defense, in particullarity, glutathione peroxidase, glutathione reductase activity and content of reduced glutathione in the rat's blood serum on 14th days of the experimental periodontitis development, and after correction with flavonol quercetin, that was injected in doze 100 mg / kg for 7 days (from the 7th to the 14th day). The results were statistically processed using parametric and nonparametric statistical methods. The reliability of the differences in values between independent quantitative values was determined with a normal distribution according to the Mann-Whitney U criterion. The article shows a decrease enzymic activity of the glutathione system (glutathione peroxidase and reductase) observed at early stage of the inflammatory process with following preservation of their activity at reduced level. At the same time, content of reduced glutathione was decreased. The introduction of quercetin resulted in the increase of enzymes in the serum, and reduced glutathione and enzymes activity in the blood serum of experimental animal with periodontitis as compared to the indices of animals that were not administered this medicine. In addition, quercetin also contributed to the preservation of a pool of reduced glutathione in the animal blood. The development of the inflammatory process of the periodontal complex due to the combined effect of bacterial and immune factors is accompanied by dynamic changes in the activity of the glutathione system. Quercetin effectively stabilizes the prooxidant-antioxidant system during the course of experimental periodontitis, which is manifested by the increased activity of glutathione peroxidase and glutathione reductase and the increase in the content of reduced glutathione.