Cardioprotectoral Influence of Metabolitotropic Measures in Physical Loading of Intact Rats and on the Coronary Vasospasm

Physical loading during training exercises and competitions may influence the organism' adaption ability, including the cardiovascular system activity. More prominent, cardiac metabolism and function may be changed in the coronary spasm condition with the physical loading. The purpose of the study was to establish the metabolitotropic compounds on the capacity of intact rats and in the conditions of pituitrine coronary vasospasm, ECG and metabolism of myocardial markers. Material and methods. Experiments were conducted on white male rats without line. The physical loading was modulated by running on tredbane with the velocity of ribbon 50 sm / min till exhaustion. There was an experimental series when before the physical loading pituitrine had been administered in dose 1 Unit/kg intraperitoneally for coronary vasospasm modulating. The biochemical experiments were created according to the Methodical recommendation of state Expert Center of the Ministry of Health of Ukraine. The changes of ST segment at ECG and level of ATP, markers of oxidative proteins damage in the rats' hearts were investigated. The heart tissue was gomogenased in cold acidic isotonic environment (0,15 M KCl) in temperature 4°C by glass gomogenasor. Then the gomogenated tissue put into HCl (0,6 M) to obtain extract without proteins. The drugs have been introduced intraperitoneally in 30 minutes before moving hypoxia modulating in intact rats or before pituitrine administration. Angiolin was introduced in dose 50 mg/kg. mildronate, yakton, mexidol in the dose 100 mg/kg. The rats were decapitated under thiopentalum-natrium injection. Statistic investigations were made with the programs «STATISTICA for Windows 6.0» and «Microsoft Excell 2003». Results and discussion. After the modeling moving hypoxia it was stated in the rats' hearts decrease of ATP on 27,5%, increase ∑ΔST on ECG in 48 times, the level of the markers of oxydative damage of proteins – APhG on 65.%, KPhG on 93.7%. The development of oxidative processes and deficiency of energy was shown. Angiolin increased the duration of running by 53.5%, level ATP on 28%, decrease the content of APhG by 38.3%, KPhG by 44.2%. All other methabolitotropic drugs – mildronat, yakton, mexidol influenced on the biochemical data changes in the same development but in less degree. Conclusions. The more disturbances in heart activity and biochemical markers were stated in the conditions of pituitrine coronary vasospasm. The following results were obtained: the decrease of running duration by 71%, ATP level in 3,4 times, elevation of ∑ΔST on 112%, content of APhG in 2,28 times, KPhG in 2,68 times. Angiolin introduction raised the tolerance to physical loading – running duration by 70%, ∑ΔST on ECG by 63%. Angiolin increased ATP level at 37% in the heart, and decreased the content of oxidative damage of proteins markers – APhG by 30%, KPhG by 39%. Angiolin introduction has cadioprotective and adaptogenic action in physical loading and it cooperates with pituitrine coronary vasospasm. Other metabolititopic drugs changed ∑ΔST and biochemical markers in physical loading and its cooperation with pituitrine coronary vasospasm in the same direction.