Study of Intensity of Lipids Peroxidation, Content of Vitamin C, Activity of Succinate Dehydrogenase in Adrenal Cells of Rats in Conditions of Experimental Nephropathy and Introduction of Exogenous Glutathion

The topicality of the study of changes in the adrenal glands in conditions of experimental nephropathy is determined by the influence of glucocorticoid hormones on the metabolic processes of the organism and their possible role in the regulation and complication of the pathological process. Protection against oxidative stress in the body is carried out by various antioxidants, one of them is glutathione. Glutathione serves several vital functions including antioxidant defense, detoxification of xenobiotics and their metabolites, regulation of cell cycle progression, maintenance of redox potential, modulation of immune function. As oxidative stress and energy imbalance accompany many illnesses, the purpose of the work was to investigate the intensity of spontaneous and ascorbate-dependent lipid peroxidation, the content of vitamin C in the adrenal glands, and changes in succinate dehydrogenase activity in adrenal mitochondria by experimental nephropathy and glutathione introduction. Material and methods. The experiment was carried out on male albino rats with the body weight 0.16-0.18 kg. The animals were introduced a single intraperitoneal dose of folic acid (250 mg/kg) for modeling nephropathy. Exogenous glutathione was introduced intragastral (100 mg/kg) during 3 days after intoxication with folic acid. The isolation of the mitochondrial fraction was performed by differential centrifugation. The intensity of spontaneous and ascorbate-dependent lipid peroxidation in rat adrenal mitochondria was evaluated by the formation of malonic dialdehyde by the color reaction with thiobarbituric acid. The content of vitamin C was determined by the ability to eliminate 2.6-dichlorophenolindophenol. Succinate dehydrogenase activity was determined by the amount of reduced ferricyanide. Results and discussion. Results were estimated using nonparametric T-Wilcoxon criterion. The level of significance was P < 0.01. It was shown that under conditions of experimental nephropathy, the rate of formation of TBA-active products increased both spontaneously and ascorbate-induced. The content of ascorbic acid in the adrenal homogenate of rats experimental nephropathy decreased. At the same time, in the mitochondria of the adrenal glands of experimental animals with pathology, the level of TBA active products increased and succinate dehydrogenase activity was suppressed. Conclusions. Our results demonstrate that introduction of glutathione during 7 days normalized the content of ascorbic acid in the adrenal homogenate of rats with experimental nephropathy. The rate of peroxidation of lipids in the adrenal homogenate, the content of TBA-active products, and the succinate dehydrogenase activity in the adrenal mitochondria of animals with experimental nephropathy under influence of glutathione approximated the values of the intact group. Perhaps the tripeptide may slow or stop the progression of kidney disease and its complications