Evaluation of the Susceptibility of Trichomonas vaginalis Isolates to Melaleuca cajuputi

Trichomonosis is the most common nonviral sexually transmitted disease (STD) in the world. Due to the development of metronidazole-resistant isolates, therapeutic alternatives to 5-nitroimidazole are being investigated. T. vaginalis can be naturally infected with Mycoplasma hominis, Mycoplasma girerdii and Trichomonas vaginalis virus (TVV). Objective: In this research, the essential oil from plant Melaleuca cajuputi used to test the anti-trichomonas activity. Meanwhile, symbiosis was checked to determine whether any correlation between sensitive and nonsensitive T. vaginalis isolates with essential oil. Materials and methods: A total of 32 T. vaginalis isolates from Hue province and Sassari University subjected to susceptibility testing against essential oils by broth microdilution method. Polymerase chain reaction (PCR) was used to detect the presense of M. hominis and M. girerdii. TVV haboring protozoan was identified by total RNA extraction. Result: The M. cajuputi essential oil showed antitrichomonal activity at the mean of MIC at 0.08±0.05% after 24 hours and 0.06±0.05% after 48 hours . There was no significant differences in MIC of EO to T. vaginalis-microorganism-infected and T. vaginalis-free. Conclusion: M. cajuputi essential oil can be u