Method Development and Validation of Atenolol Drug by Spectrophotometric and HPLC Technique in Forensic Application

Atenolol is a selective 1 receptor antagonist. The drug belonging to class of beta blockers used primarily in hypertension and cardiovascular diseases. It is highly misused drug and overdoses lead to death. The aim of this work was to develop a simple, isocratic, precise, accurate and sensitive Spectrophotometric and HPLC technique for determination of Atenolol. The HPLC technique is costly, caring and complicated so the applied method is novel, safe and sensitive for spectrophotometric estimation in UV-region. Then calibration curve was prepared in chosen solvents. The suitability, accuracy and precision studies were done by calculating SD and %RSD. The recovery of Atenolol was found near to 99.5 %. Validation of proposed methods was carried out for its suitable conditions according to guidelines and these methods are scaled up in pharmaceutical and forensic application.