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GENOME ANALYSIS OF Pseudomonas brassicacearum S-1 – AN ANTAGONIST OF CROP PATHOGENS

Journal: Biotechnologia Acta (Vol.14, No. 2)

Publication Date:

Authors : ;

Page : 47-58

Keywords : genome; sequencing; Pseudomonas brassicacearum; secondary metabolites; biocontrol.;

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Abstract

The strain Pseudomonas brassicacearum S-1 is the basis of the biopesticide “Ecogreen”, which is used to control pathogens infecting vegetable and green spicy crops in small-scale hydroponics. Aim. The purpose of this work was to sequence and analyze the nucleotide sequence of the genome of strain P. brassicacearum S-1 (GenBank accession number CP045701). Methods. Whole-genome sequencing was performed by both MiSeq (Illuminа) and MinION (Oxford Nanopore). Analysis of the genome sequence was performed with a number of bioinformatics programs. Results. The genome of the P. brassicacearum S-1 strain comprising a single circular 6 577 561-bp chromosome with GC content of 60.8 %. Genome analysis revealed genes that constitute valuable biotechnological potential of the S-1 strain and determine synthesis of a wide range of secondary metabolites. Moreover, mobile genetic elements, prophages and short repetitive sequences were identified in the S-1 genome. Conclusions. Detected genetic determinants, which are responsible for the synthesis of practically valuable compounds, indicate a significant potential of the P. brassicacearum S-1 strain as a biocontrol agent.

Last modified: 2021-09-15 23:57:58