GENOME ANALYSIS OF Pseudomonas brassicacearum S-1 – AN ANTAGONIST OF CROP PATHOGENS
Journal: Biotechnologia Acta (Vol.14, No. 2)Publication Date: 2021-04-30
Authors : A. A. Muratova A. E. Akhremchuk L. N. Valentovich;
Page : 47-58
Keywords : genome; sequencing; Pseudomonas brassicacearum; secondary metabolites; biocontrol.;
Abstract
The strain Pseudomonas brassicacearum S-1 is the basis of the biopesticide “Ecogreen”, which is used to control pathogens infecting vegetable and green spicy crops in small-scale hydroponics. Aim. The purpose of this work was to sequence and analyze the nucleotide sequence of the genome of strain P. brassicacearum S-1 (GenBank accession number CP045701). Methods. Whole-genome sequencing was performed by both MiSeq (Illuminа) and MinION (Oxford Nanopore). Analysis of the genome sequence was performed with a number of bioinformatics programs. Results. The genome of the P. brassicacearum S-1 strain comprising a single circular 6 577 561-bp chromosome with GC content of 60.8 %. Genome analysis revealed genes that constitute valuable biotechnological potential of the S-1 strain and determine synthesis of a wide range of secondary metabolites. Moreover, mobile genetic elements, prophages and short repetitive sequences were identified in the S-1 genome. Conclusions. Detected genetic determinants, which are responsible for the synthesis of practically valuable compounds, indicate a significant potential of the P. brassicacearum S-1 strain as a biocontrol agent.
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