THE CONTENT OF PHENOLIC COMPOUNDS AND FLAVONOIDS IN Deschampsia antarctica TISSUE CULTURE
Journal: Biotechnologia Acta (Vol.14, No. 2)Publication Date: 2021-04-30
Authors : M. O. Twardovska I. I. Konvalyuk K. V. Lystvan I. O. Andreev V. A. Kunakh;
Page : 59-66
Keywords : plant tissue culture; Deschampsia antarctica E. Desv.; phenolic compounds; flavonoids; HPLC analysis.;
Abstract
Aim. The aim of the study was to determine the quantitative and qualitative content of phenolic compounds and flavonoids in Deschampsia antarctica E. Desv. tissue cultures obtained from plants originating from different islands of the maritime Antarctic. Methods. In vitro tissue culture, Folin-Ciocalteu method, spectrophotometry, HPLC analysis. Results. The quantitative content of phenolic compounds and flavonoids in D. antarctica tissue cultures obtained from plants of six genotypes (DAR12, DAR13, G/D12-2a, Y66, R30 and L57) was determined. The highest content of phenolic compounds (4.46 and 3.75 mg/g) was found in tissue cultures obtained from root and leaf explants of plant genotype L57. The highest amount of flavonoids (7.17 mg/g) was accumulated in G/D12-2a tissue culture of root origin. The content of the studied biologically active compounds (BACs) did not change with increasing number of subculture generations (from passage 10 to 19). HPLC analysis showed that in D. antarctica tissue cultures, a shift in the biosynthesis of BACs occurred towards the synthesis of more polar metabolites compared to explant donor plants. Conclusions. It was found that the transition of cells to undifferentiated growth affected the content of BACs, the amount of which decreased 2β5 times simultaneously with a significant change in their profile. This provided a basis for further biochemical studies, as well as for careful selection of tissue culture of D. antarctica to use it as a potential source of BACs.
Other Latest Articles
- Pelatihan Kewirausahaan dan Pemasaran bagi Pengurus Koperasi Usaha Kecil dan Menengah di Kelurahan Tugu Cimanggis
- GENOME ANALYSIS OF Pseudomonas brassicacearum S-1 β AN ANTAGONIST OF CROP PATHOGENS
- ASSESSMENT OF GENETIC STRUCTURE VARIABILITY OF RAINBOW TROUT Oncorhynchus mykiss (WALBAUM, 1792) OF UKRAINIAN LOCAL STOCKS USING POLYMORPHIC BLOOD PLASMA PROTEINS
- APPLICATION EFFICIENCY OF BOVINE SERUM ALBUMIN FOR RECOVERY OF SEMINIFEROUS TUBULES OF TESTES AFTER CRYOPRESERVATION
- Pelatihan Pemasaran Syariah Berbasis Digital bagi Pelaku UMKM di Kelurahan MekarsariCileungsi
Last modified: 2021-09-15 23:59:53