Comparative Evaluation Of Second Generation Platelet Concentrates On Fibroblast Cell Viability And Migration

Objective: The aim of this study was to compare the effect of L-PRF, A-PRF, A-PRF+ on fibroblasts by evaluating the viability and migration of cells. Methods: Peripheral blood samples were collected from 3 healthy volunteers (3 from each individual for L-PRF, A-PRF and A-PRF+). Following respective centrifugation protocols L-PRF (2700 rpm for 12min), A-PRF (1500 rpm for 14min), APRF+ (1500rpm for 8min) membranes were obtained. 3T3 fibroblast cell lines were treated with each membrane and cell viability was assessed by MTT assay. Fluorescent images were captured and quantified with an inverted fluorescent microscope. Cell migration was assessed by scratch wound healing assay at intervals of 0, 24, 48 and 72hrs. Wound closure was analysed under the inverted microscope. Results: At 24 hrs, no statistically significant differences were noted with regard to cell viability among A-PRF, A-PRF+ and L-PRF groups (98.33±4%, 99.18±4% and 99±2%, p>0.05 ). Within the groups there were significant differences in cell migration, from 0 hrs to 24, (p<0.05), 24 to 48hrs (p<0.001) and 48 to 72 hrs (p<0.01). Significant differences were also noted between L-PRF and A-PRF at all time intervals while closing the cell free scratch area. Conclusion: The results from the present study demonstrates higher cell viability indicating excellent biocompatibility of all the platelet rich membranes on fibroblasts and L-PRF showing higher fibroblast migration over the advanced fibrins (A-PRF and A-PRF+) at early time intervals indicating rapid degranulation. Further studies with increased sample size over a longer evaluation period are required to confirm the results.