Analysis of Inhibitory Propensity and Percentage Inhibition of Tecoma stans and Plectranthus vettiveroides Leaves against Escherichia coli ESBL Enzyme in Namakkal District Broilers, Tamil Nadu, India

On samples phenotypically positive for extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli, the inhibitory potential and percent inhibition of fresh juice from Tecoma stans and Plectranthus vettiveroides leaves on beta-lactamase enzyme of cecal samples of healthy broilers were tested. Two hundred (200) samples of cecal treated for ESBL-producing E. coli were obtained from 38 Namakkal poultry selling outlets. The impacts of T. stans and P. vettiveroides leaves were interpreted as chromogenic substrate by colorimetric assay with CENTA and nitrocefin. The mean absorbance value of the inhibitory potential was inversely propotional. Tecoma stans exhibited 29% and 31% of CENTA and nitrocefin inhibition, respectively. The mean absorbance value of Plectranthus vettiveroides and the 10.0% inhibition of CENTA and nitrocefin in both (p > 0.05) showed no significant difference in CENTA and nitrocefin activity. As normal regulation, tazobactum (100 μM) exhibited a 99.89% and 98.31% inhibition against CENTA and nitrocefin, respectively. The combination of Plectranthus vettiveroides and Tecoma stans showed 14% to 15% inhibition, respectively, with CENTA and nitrocefin. Results showed that beta-lactamase enzyme activity which inhibited with T. stans was higher than P. vettiveroides leaf juice and the combination of the two herbs showed no substantial difference in activity.