ECONOMICAL BIOPROCESS DEVELOPMENT TO ENHANCE THE ALKALINE PROTEASE ENZYME PRODUCTION BY USING PENCILIUM OXALICUM KRSS-S-FP10 WITH STATISTICAL TOOLS

Alkaline protease producing microorganism's isolated total of 74 and 69 fungi from beach soils and black soils respectively were 10 from Kakinada beach soil and 9 from Vishakhapatnam beach soil and 40 isolates from Tenali black soil. The best producing organism identified and characterized as Pencilium oxalicum KRSS-S-FP10. Taguchi was used to know active and independent variables screened by using PDA media variables was employed to derive statistical model (Response surface methodology: RSM) for optimizing the medium composition. The alkaline protease activity was in between 2.0U/mL to 10.76U/mL and The Delta value indicates the effect of component in the form of ranking has given to the variables. To confirm these results, experiments were carried out using RSM these nutrients with same concentrations and it was observed that the mean value of alkaline protease was 10.13U/mL as compared to the predicted value of 10.76U/mL using MINITAB. The final optimized medium has given approximately three-fold increase in alkaline protease production in comparison with one-factor-at-a-time (5.12U/mL) method.