The Antioxidant Activity Test with DPPH and Antibacterial Method with Minimum Inhibitory Concentration Method of Andaliman Fruit (Zanthoxylum acanthopodium DC.)

The utilization of bioavailability, according to the World Health Organization (WHO) records, is extensive; it is estimated that nearly 80% of humanity, especially in developing countries, still uses plants as medicinal materials to maintain their health. This experimental research method includes collecting and processing andaliman fruit, making ethanol extracts, and examining antioxidant activity testing using the DPPH (1,1-diphenyl-2-picrylhydrazil) free radical capture method and antibacterial activity using the minimum inhibitory concentration method. The research was conducted at the Pharmacognosy Laboratory and Research Laboratory, Faculty of Pharmacy, University of North Sumatra. The study was conducted in November - December 2021 for sampling and examination. Ethanol extract from andaliman fruit is a sample with extreme antioxidant activity because it has an IC50 value with a concentration of 12.5- 100μg/mL and potent antioxidant activity with an IC50 value with a concentration of 25-100 μg/mL. Ethanol extract from andaliman fruit can inhibit Staphylococcus aureus and Staphylococcus epidermidis, effective at a concentration of 300 mg/ml with an inhibition diameter of 10.17 mm and 10.80 mm.