Effect of caffeine-loaded silver nanoparticles on minerals concentration and antibacterial activity in rats

Caffeine boosts metabolism and the neurological system. When extreme weakness or sleepiness occurs, it is used recreationally and medicinally to reduce physical and mental fatigue. Caffeine initially stimulates the central nervous system, increasing intellect, speed, accuracy, focus, and coordination. The aim of the study was to evaluate how caffeine nanoparticles affect potassium, calcium, zinc, and magnesium levels, in addition, the anti-bacterial activity of the samples has been measured. Eighteen male albino rats were divided into 3 separate groups. The first group (G1) was made up of 6 animals that served as a control group. The second group (G2) was made up of 6 animals that were given caffeine, and the third group (G3) was made up of 6 animals that were given silver nanoparticles from a caffeine solution. The particle size and structural morphology of caffeine and silver nanoparticles were analyzed using Brookhaven Instruments Corp., XRD and SEM, respectively. The structural results showed that after addition, caffeine was tube-shaped, and silver was spherical granular. Caffeine has more silver nanoparticles than caffeine solution. Caffeine solution affects potassium, calcium, zinc, and magnesium levels. Additionally, the solution has antibacterial activity against the following bacteria: Staphylococcus aureus (Gram +ve), E. coli (Gram –ve), and Pseudomonas aeruginosa (Gram-ve), but it has no effect against yeast (Candida albicans). It is concluded that caffeine-synthesized Ag NPs has significant biological effects on zinc, magnesium, calcium, and potassium levels in male albino mice and this also is antibacterial against Staphylococcus.