New Quantitative PCR Technology for Screening Human Papillomavirus Test – Identification of 25 Genotypes: A Molecular Epidemiology and CrossSectional Study |Biomedgrid

Background A kit for screening HPV-genotypes based on the prevalence of Mexican population there isn´t designed Objective To design a new molecular test for human papillomavirus (HPV) screening. Methods Molecular epidemiology and cross-sectional study. Multicenter from referral centers. 326 women were included. The primary outcome was designed of a kit for screening HPV-genotypes based on the prevalence found in population. Oligonucleotides for different genotypes were designed. The conditions for quantitative PCR and the pools for low- and high-risk (LR and HR) of HPVgenotypes were established.