Discovering of Peptide Contributions to Drug Developments

Rusticyanin, a protein of 155 amino acids obtained from Thiobacillus ferrooxidans was used to design RP131-155 peptide (Mw=2688.21). The aim of this study was to investigate the antimicrobial effect of various concentrations of RP131-155, a peptide derived from the N-terminal of rusticyanin on Enterobacter aerogenes, Salmonella arizonae, Escherichia coli and Staphylococcus aureus. The antimicrobial activity was determined by using disk diffusion method. A higher inhibition zone was observed for E. coli, E. aerogenes, S. arizonae and S. aureus respectively. The minimum inhibition concentration (MIC) value of RP131-155 against E. coli, E. aerogenes and S. arizonae was shown to range between0.08-0.16mg/ml whilst that of S.aureus was between 0.32- 0.63mg/ml