BACTERIOLOGICAL STUDY AND MOLECULAR CHARACTRAIZATION OF PORPHYROMONAS GINGIVALIS ISOLATED FROM PERIODNTIIS PATIENTS IN HILLA CITY, IRAQ

One hundred three clinical samples were collected from subgingival dental plaque of patients with chronic (CP) and aggressive periodontitis (AP) (72 and 31) respectively, admitted to teaching Hospital in college of Dentistry / Babylon University. These dental plaque samples were subjected to different methods for identification of P. gingivalis mainly traditional bacteriological method. It was found that 23 P. gingivalis isolates were recovered by using selective media where 19 isolates (26.3%) obtained from (CP) and 4 isolates (12.5%) from (AP). Molecular detection method was applied by using 16s rRNA gene as a genetic marker for confirmation of detection of P. gingivalis isolates, 12 isolates of P.gingivalis out of 23were detected by molecular method focusing on the role of 16s rRNA gene of P. gingivalis. 8 isolates was isolated from CP, and 4 isolates were isolated from AP subgingival plaques. The screening of antimicrobial activity of (CHX) gluconate (0.12%) was carried out and the results showed that it produced the highest inhibition activity whether against p. gingivalis with inhibition zone range 40-15mm. Finally in vitro antibacterial activity of Alum, Clove and Cyperus rotundus plant extracts was studied and the results revealed that all tested isolates were inhibited by aqueous extracts at 50% concentration. The maximum inhibition zone was observed in Alum and Clove extracts respectively (30mm, 25mm), compared to the minimum inhibition by C.rotundus (10mm). The antibacterial actions of 20% concentration of plant extract gave lower inhibition zone than 50% concentration of that extract which represented by (19mm, 16mm, and 10 mm) of the previous three mentioned extracts.