Development and adaptation of a biosensor based on lactate oxidase and poly-m-phenylenediamine for the determination of L-lactate in real biological samples

Aim. Development and optimization of an amperometric biosensor based on lactate oxidase with a poly-meta-phenylenediamine membrane for the accurate measurement of L-lactate in blood serum. Material and Methods. The biosensor was based on a platinum disk amperometric transducer modified with a PPD membrane with immobilized LOx. Measurements were performed in a threeelectrode amperometric system at a potential of +0.6 V. The real sample results were verified spectrophotometrically and by an electrochemical analyzer. Results. The biosensor demonstrated a wide linear range (7–1000 μM), a low detection limit (7 μM), and high selectivity. The standard addition method showed a high correlation (0.982) with reference methods. Conclusions. The developed biosensor is promising for clinical diagnostics of blood lactate levels.