MICROSCOPICAL OBSERVATION OF TESTES FOR AMELIORATIVE EFFECTS OF VITAMIN - E & C IN STREPTOZOTOCIN INDUCED DIABETIC RATS

Oxidative stress induces many serious reproductive tissue damages in male and female gonads and thus poses a serious threat to reproductive health. Lipid peroxidation is an important biological consequence of oxidative cellular damage in patients with DM. Serum lipo-peroxidation products such as malondialdehyde (MDA) reflects oxidative stress. The purpose of this study is to evaluate the effects of Vitamins E and C (VEC) on diabetic rat in prevention of Streptozotocin induced damages on testis. The experiments were performed on twenty four (n = 8) male Albino rats (approx 200-250 g) obtained from Animal house, National Medical College, Birgunj, Parsa, Nepal. The twenty four male albino rats were divided into three groups (n = 8) as Group-A: Control group with normal diet (Saline solution, i.p.); Group-B: Diabetic group with Streptozotocin; The rats developed diabetes after injection was evidenced by sustained hyperglycemia and glycosuria seven days after the induction; Group-C: (Diabetic + VEC) Albino rats were induced diabetes mellitus with Streptozotocin are evidenced by sustained hyperglycemia and glycosuria after seven days, and then it was treated with VEC supplementation for seven days. The experiment was conducted for a period of five weeks. After the experimental period the serum testosterone and tissue malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH) levels were measured, and testis tissue was examined histopathologically. Elevated malondialdehyde and reduced superoxide dismutase, glutathione, and serum testosterone levels were detected. Histopathological observation detected that marked changes in the testis tissue in all the groups. Our results provide additional evidence for the concept of increase in oxidative stress by diabetes mellitus causes free radical damage in testis tissue. An improvement in this damage can be achieved by vitamin E & C treatment.