ANALYSIS OF THE RATS' LIVER BINUCLEATE HEPATOCYTES VOLUME CHANGE UNDER THE INFLUENCE OF OPIOIDS DURING THE THREE WEEKS EXPERIMENT

Aim. To analyze the morphological changes of the rat liver, particularly the changes of the volume of binucleate hepatocytes during the experiment. Materials and Methods. White male rats, 190-230 gr. of weight, were selected for the study; two randomized groups of animals were formed: ? - the control group of rats, ?? - the experimental group (receiving intramuscular injections of opioid "Nalbufin"). The dose of the opioid was increased every week. "Nucleator" method was applied to determine the volume of hepatocytes. Results and Discussion. It was determined that the hepatocytes volume varies in different weeks of the experiment. The median indices testify that the hepatocytes volume decreases in the 3- rd week (1697 μm3), while in the 1st and 2nd weeks the cell volume increases to 3157 μm3 and 3479 μm3 respectively. The value of standard deviation in the 1st week was close to the control - 2672, the maximal on the 2nd week - 3833, while the minimal on the 3rd week - 2221, respectively. The maximal mean of the volume of the cells was revealed in the 2-nd week (4539 μm3) of experiment, while the decrease of the mean of the cell volume occurred in the 3rd week - 2338 μm3. Conclusion. Thus, according to the data, the results of our investigation show the dynamics of the volume of the binucleate hepatocytes during all time periods of the experiment. The volume of the cells reaches its maximum in 2nd week, and its minimum - in 3rd week. Key words: opioids, binucleate hepatocytes, nucleator, liver