SYNTHETIC APPROACH TOWARDS PYRIMIDO [4, 5-B] QUINOLINE WITH 2-SUBSTITUTED DERIVATIVES AND STUDY OF ANTIMICROBIAL ACTIVITY

2-Amino-8-nitropyrimido[4,5-b]quinoline (1) and ethyl 2-cyano-3,3-bis(methylthio)acrylate (2) were refluxed in N,N-dimethyl formamide (DMF) in presence of catalytic amount of anhydrous potassium carbonate to afforded 3-cyano-8-nitro-2- (methylthio) -4-oxo- 4H- pyrimido [2,1-b] pyrimido [4,5-b] quinoline (3). The latter were further reacted with different substituted aniline, phenol, hetryl amine and compound containing active methyl groups. Afforded to 3-cyano-8-nitro- 4- oxo- 4H- pyrimido [2, 1-b] pyrimido [4, 5-b] quinoline and their 2-substuited derivatives (4a-7c). All these newly synthesized compounds were characterized by elemental analysis and spectral data, and screened for their antimicrobial activities.