Development and Validation of HPTLC Method for Estimation of Saroglitazar in Bulk and Pharmaceutical Dosage Form

A simple, sensitive, and precise high-performance thin-layer chromatographic method has been developed for the estimation of Saroglitazar (SAR). The method employed was thin-layer chromatography (TLC). Aluminum plates precoated with silica gel 60 F254 were used as the stationary phase, while the solvent system was n-butanol: ammonia (7:3 v/v). The Rf value was observed to be 0.55 ± 0.02. The spot was densitometrically analyzed at 294 nm. The method was linear in the range of 125-1500 ng/ band. The limit of detection for SAR was found to be 13.09 ng/ band. The limit of quantification for SAR was found to be 39.68 ng /band. The proposed method was validated with respect to linearity, accuracy, precision and robustness. The method was successfully applied to the estimation of SAR in formulation.