Novel Approach towards Synthesis of Silver Nanoparticles from Myxococcus virescens and their Lethality on Pathogenic Bacterial Cells

Aim: We report extracellular synthesis of silver nanoparticles by cell filtrate of Myxococcus virescens. Moreover, the effect of silver nanoparticles on survivability or lethality of human pathogenic bacteria viz. Staphylococcus aureus (ATCC 6538), Bacillus subtilis subsp. spizizenii (ATCC 6633) and Pseudomonas aeruginosa (ATCC 10145) has been studied. Methods and Results: The silver nanoparticles were synthesized from M. virescens cell filtrate, and characterized by UV-Vis Spectroscopy, Nanoparticle Tracking Analysis (NTA) by LM 20, Zeta Potential analysis, Fourier Transform Infra-Red Spectroscopy (FTIR) and Transmission Electron Microscopy (TEM). Antibacterial efficacy of silver nanoparticles was tested which comprised the effect of survivability or lethality of human pathogenic bacteria. Biosynthesized silver nanoparticles demonstrated promising cell lethality for human pathogenic bacteria. Conclusion: M. virescens mediated synthesized silver nanoparticles are quite stable, and capped with proteins. This is a sustainable, eco-friendly and simple process for synthesis of desirable silver nanoparticles which is havinglethal properties against some clinical bacterial cell. Significance and Impact of Study: M. virescens mediated synthesized nanoparticles could make a starting point to obtain new antibacterial substances towards multiple antibiotic-resistant pathogenic bacteria.