Isolation and identification of medicinal compounds from Kalanchoe pinnata of Crassulaceae family by 1H NMR

The details of phytochemical and biological investigations of Kalanchoe pinnata, belong to the Crassulaceae family grown in Bangladesh has not been studied extensively. Here, Kalanchoe pinnata has been investigated for the isolation of its secondary metabolites by vacuum liquid chromatography. The powdered leaf of Kalanchoe pinnata was extracted with ethanol. The concentrated extract was partitioned with n-hexane, carbon tetrachloride and Dichloromethane. Chromatographic fractionation and purification of n-hexane partitionate provided two compounds. These two isolated compounds were identified by extensive analyses of their high resolution 1H NMR spectral data. The purified compounds were campesterol and 5,6,7,8,4' pentahydroxy flavanone. This is the first report of their occurrence in Kalanchoe pinnata.