Callus culture of Azadirachta indica employing its stem

Azadirachtin,an insecticidal active ingredient present in neem tree i.e.Azadirachta indica A. Juss.seeds and they varies considerably due to environmental factors also for genetic reasons. Furthermore, the tree does not grow in moderate climates and their germination ratio is very less. It seems appropriate, therefore, to employ tissue culture techniques for the production of Azadirachtin in order to obtain constant amounts of standardized quality. Here we try to create callus, an unspecialized, unorganized, growing and dividing mass of cells through which mass cultivation further initiate. The best media generally we use is MS Media along with different concentrations of hormones i.e. Auxins(BAP) and Cytokinin (Kinetin), employing Azadirachta indica stem. Saurish Sarkar"Callus culture of Azadirachta indica employing its stem" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-2 | Issue-3 , April 2018, URL: http://www.ijtsrd.com/papers/ijtsrd10911.pdf http://www.ijtsrd.com/biological-science/biotechnology/10911/callus-culture-of-azadirachta-indica-employing-its-stem/saurish-sarkar