Potential Regeneration Capacity of Jatropha Curcas L Leaf Explants after Chemical Treatment for Aseptic In vitro Regeneration

Experiments were conducted to asses the potential tolerance of leaf explants of Jatropha curcas L to chemical components that are used during sterilization of explants for invitro regeneration resulting in generation of callus followed by shooting and rooting. Initially the seeds were sown in 96 well trays for the generation of plantlets in the green house using coco peat as the nutrient medium. Later leaf was selected as explant for invitro regeneration and was subjected to sterilization procedure. It was observed that, the leaf explants treated with 0.1 % Mercuric chloride were devoid of contamination but resulted in chlorosis. However in almost all the explants, a part of the explant still responded on callus inducing media CI-1 ( (MS+2.2M BAP + 4.9M IBA) resulting in generation of callus. But when these calli were transferred to shooting media SR-1 ( (MS+8.9M BAP + 4.4M IBA) resulted in generation of embryoids. Further these embryoids were sub cultured on SR-1 to develop in to shoots. Later these shoots resulted in rooting when transferred to half strength MS medium along with 4.4M IAA.