Analytical Method Development and Validation Description for Quantitative Assessment of Chlorbenzuron by HPLC Procedure

A consistent, systematic, exact and precise high performance liquid chromatographic method for the analysis of pesticide Chlorbenzuron in its formulations was developed and validated in the present study. The mobile phase consists of a mixture of acetonitrile, water and 1, 4 dioxane in the proportion 55: 45: 0.03 respectively (v/v/v). This was found to give sharp peak of Chlorbenzuron at a run time of 20 min. HPLC analysis of Chlorbenzuron was carried out at a wave length of 250 nm with a flow rate of 1.4mL/min. The linear regression analysis data for the calibration curve showed a good linear relationship with a regression coefficient 0.999 in the concentration range of 25% to 125%. The linear regression equation was y= 1866x+760.7. The developed method was employed with a high degree of precision and accuracy for the analysis of Chlorbenzuron. The method was validated for accuracy, precision, robustness, ruggedness and specificity. The precision, accuracy, sensitivity, short retention time and composition of the mobile phase stipulated that this method is applicable to the evaluation of Chlorbenzuron.