COMPARATIVE EVALUATION OF AQUEOUS EXTRACT AND PROTEASE FROM ADHATODA VASICA FOR DETERMINING ITS IMMUNOSUPPRESSIVE ACTIVITY

In order to compared its immunopharmacological activity of protease (against Casein) and aqueous leaves extract of Adhatoda vasica (using phosphate buffered saline, PBS, pH 7.2) against hepatitis B Vaccine containing surface antigen (HBsAg; 20 ?g/ml) and determined antibody (IgG) production through indirect Elisa and also examined its proliferative response including nitric oxide production. For these studies, qualitative assays were determined and evaluate the presence of secondary metabolites in aqueous leaves extract whereas concentration of protein is also determined for protease separation. The results showed that aqueous leaves extract and protease at higher doses showed enhancement in anti-HBsAg IgG titre as compared to standard and control but there is sudden decline in proliferation including NO production containing HBsAg at higher doses. In short, protease and aqueous leaves extract of Adhatoda vasica could be a potent immune enhancer of B cells and inhibitor of T cells. Key words: Adhatoda vasica; aqueous extract; protease; nitric oxide; proliferation; Elisa