Assembly of Gold-Binding Proteins for Biomolecular RecognitionJournal: Austin Journal of Biosensors & Bioelectronics (Vol.1, No. 1)
Publication Date: 2014-10-14
Authors : Zareie HM; Sarikaya M;
Page : 1-4
Keywords : Gold-Binding Protein (GBP); Self-assembly; Atomic Force Microscopy (AFM);
Controlled binding and assembly of proteins onto inorganics is at the core of biological materials science and engineering with wide ranging applications. Here we demonstrate ordered assembly of genetically-engineered inorganicbinding polypeptides on an atomically-flat solid surface. We used a 3-repeat, 14 amino acid Gold-Binding Protein (GBP1) that forms a monolayer-thick film with nano structured domains on the gold surface. The protein film conforms into 3-fold symmetry, commensurate with Au (111) lattice, suggesting crystallographic recognition. The GBP1 was selected for its specific affinity to gold by using cell-surface display. The engineered proteins could have significant potential impact by providing self-assembled functional molecular substrates in nanoand bio-technologies. We demonstrate directed assembly of ferritin (target) onto streptavidin (probe) conjugated to biotinylatedGBP1, and quantitatively analyze the results.
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