IMMUNOHISTOCHEMICAL CHARAC-TERISTIC OF PROSTATE CANCER TIS-SUE WITH INTRALUMINAL INCLUSIONS

Prostate cancer (PC) is the second pathology among cancers in the male population and the most common oncological disease in developed countries. PC has polyethiological reasons of occurrence: apart from age, genetic predisposition and race, its occurrence causes include the presence of pathological inclusions in the lumen of the glands of prostate. The aim of the work is to define the immunohisto-chemical status of PC with the presence and absence of in-traluminal inclusions. Materials and methods. All patients with PC with intraluminal inclusions were included into the first group (30 persons). The second group (30 persons) included patients, who had PC without signs of mineralization and corpora amylacea presence. An immunohistochemical study of ex-pression of androgens receptors (AR), p53 protein, heat shock proteins HSP 70 and HSP 90, matrix metalloproteinase 1 (MMP1), vascular endothelial growth factor (VEGF) was performed in both groups tissue. Data processing was carried out with the help of applied statistical methods, using Microsoft Excel 2010 with the addition AtteStat 12.0.5. The research results were checked for normal distribution by Shapiro-Wilk test. Evaluation of statistical significance of results difference between groups of samples was performed using nonparametric Mann-Whitney test and parametric t- Student test. The results were considered statistically significant at the probability of 95% (p <0.05). Research results. The first group tissue samples were characterized by glands cystic transformation, by the inflammatory infiltration and sclerosis development, the phenomenon of secretion stagnation in the glands. Mechanical damage of tumor cells and surrounding tissues led to their deformation, epithelium desquamation and microdermabrasion of connective tissue and tumor structures, resulting to the chronic inflammation progression. The difference between the results of an immuno-histochemical study of the expression of AR, p53, HSP 70 and MMP1 in PC samples of the groups with presence and absence of intraluminal inclusions was not detected (p> 0.05). HSP 70- and HSP 90-positive proteins were found in the structure of corpora amylacea, which indicates their involvement in the formation of these pathological intraluminal inclusions. In general, evaluating the influence of intraluminal inclusions on the PC course, it can be noted that they contrib-ute to maintaining of the chronic inflammation of the tumor and surrounding tissues by mechanical damage.