HEPATOPROTECTIVE ACTION OF Artemisia pallens lEAVES ON Pangasius Sp. TREATED WITH CHLOFIBRATE AND PHENOL

Aim. The key motive was to investigate the toxicological impact of clofibrate and phenol prescribed under human medicine, having potential in water and sediments contamination via input from sewage treatment plants as active pharmaceutical compounds discharge into the environment had kindled present harmful effects on the aquatic ecosystem. Methods. The present study was performed on the fish model, Pangasius sp.. After acclimatization period, fishes were exposed to the appropriate LC50 concentration of the tested drugs. Their toxic effects were studied in terms of oxidative stress markers, antioxidant status, and protein damage levels in the occupancies environment under the action of bioremediation source, Artemisia pallens, and further proceeded by histopathological study and cortisol level measurements. Results. The results comparison between fish maintained under the bioremediation source, when exposed to clofibrate and phenol resulted in severe oxidative stress (significant at *P<0.001, #P<0.001, respectively) with significant alterations in antioxidant enzyme activities (significant at *P<0.001, #P<0.001, respectively), histopathological changes and cortisol levels. Bioremediation with the use of Artemisia pallens alleviated both clofibrate- and phenol-induced elevation of cortisol level (significant at *P<0.05, #P<0.05, respectively), indicating pronounced effects on the cell survival via protecting against oxidative damage. Conclusion. Determination of several biomarkers, including indices of oxidative stress and cortisol levels, may serve as a convenient approach for pollution biomonitoring of aqueous environment. Artemisia pallens water extracts can be applied as an effective bioremediation agent.