PROTEIN PATTERNS OF TISSUE AND MUCUS EXTRACT OF ARION ATER (BLACK SLUG) THROUGH UREA - SDS - PAGE P. Swapna * 1 , T. Ravinder reddy

Objective: The present investi gation has been under taken to study the elect rophoretic banding patterns of proteins in the tissue and mucus extract of Arion ater (Black slug). Methods: Ur ea Sodium dodecyl sulphate and P olyacrylamide gel electrophoresis ( Urea - SDS - PAGE) of tissue extract and mucus was separated through 12% Urea - SDS - PAGE using Silver nitrate staining. Results: The patterns of protein bands indicated a distinct pattern of four bands with weak staining were observed in the tissue extract and two in mucus extraction and one additional band with weak staining. The protei n patterns were identified by using a marker rela tive molecular weight of appro ximately 23 - 175KDa. Rm value of protein bands were calculated accordingly. Conclusion: The electrophoretogram revealed that both the protein patterns of tissue and mucus extract s showed homology in protein bands with minor variations. Key words : Arion ater, mucus, Urea SDS - PAGE, Rm value, Protein bands