IN-VITRO SCREENING OF METHANOLIC LEAF EXTRACTS OF ALBIZIA SAMAN (JACQ.)MERR, FOR THEIR ANTIOXIDANT PROPERTY
Journal: International journal of Pharmaceuticals and Health care research (Vol.02, No. 3)Publication Date: 2014-09-30
Authors : Anju Venugopal; Suresh Arumugam; Suriya Annamalai; Senthilkumar Natesan;
Page : 179-183
Keywords : Albizia saman; DPPH assay; Reducing power assay; Free radical scavenging; Antioxidant activity.;
Abstract
The present study was designed to evaluate the in-vitro antioxidant potential of methanolic extract of Albizia saman leaves. The extraction of leaves was carried out by Soxhlet apparatus using methanol. The methanolic extract investigated for free radical scavenging activity of the 1, 1-diphenyl-2-picrylhydrazyl radical (DPPH), reducing power assay and nitric oxide scavenging activity. In DPPH assay the methanolic extract at a concentration 500?g/ml was showed 79.7049±0.2158. The methanolic extracts showed maximum activity of 81.608±0.2843% at 500?g/ml in nitric oxide scavenging assay. In reducing power assay the methanolic extract showed maximum absorbance of 0.3706±0.00158 at 500?g/ml .The phytochemical screening revealed the presence of alkaloids and polyphenolic compounds. This suggest a potential utility of the plant as a source of phenolic antioxidants and may provide leads in the ongoing research for natural antioxidants form Indian medicinal plants to be used in treating diseases related to free radical reactions.
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