Phytochemical evaluation of arial part of Leptadenia reticulata (Retz) for poly phenolic compound and free scavenging activity.

Phytochemical evaluation of arial part of Leptadenia reticulata (Retz) for poly phenolic compound and free scavenging activity. Thus to determination the Total Phenolic Content (TPC) quantified by Folin?Ciocalteu method and free radical scavenging (antioxidant) activity by in LRUSM & Acetone Extract (LRA) & methanolic (LRM) and aqueous (LRAQ) extracts of Arial part of the Leptadenia reticulata (Retz),. Currently, there has been an increased interest globally to identify antioxidant compounds that are pharmacologically potent and have low or no side effects for use in preventive medicine in number of degenerative diseases.Total Phenolic content was found to be 2.77 % w/w in LRM & 1.33 % w/w in LRA eq Gallic acid and total flavanoid content was found to be 3.21% w/w eq. to quarcetin in LRM. (IC50) was calculated from the graph plotting inhibition percentage against extract concentration .indicated that the extracts (LRM, LRA & LRUSM) of L.Reticulata have notably reduced the stable free radical of DPPH (Graph 2, 3, 4 and5) to the yellow-colored Diphenyl picryl hydrazyl with an IC50 values 56.66, 55.55 and 47.20 μg/ml respectively showed in (Table 1) comparison with Ascorbic acid (Vitamin-C) (IC50 = 40.70 μg/ml) And BHA( IC50 = 61.74 μg/ml). Free radical activity of various extract of Leptadenia reticulata is due to present of polyphenolic compound present in LRUSM and LRM.