THE CELL CYCLE, DNA FRAGMENTATION CELLS THE LUNGS OF RATS DURING BURN DISEASE

The topic presents the results of the study of lung cell cycle kinetics parameters in rats, determined by method of flow DNA-cytometry with underlying thermal burn injuries to the skin and administration of 0,9 % NaCl solution on 1-st, 3-nd and 7-th days after injury. It was found that the parameters of lung cell cycle in rats with no significant burn injuries were characterized by a predominance of G0G1 cells and the balance between the processes of synthesis (S-phase) and apoptosis (SUB-G0G1 interval). The apoptosis (increase in number of cells within SUB-G0G1 interval) while maintaining the ability of cells to DNA synthesis (S-phase) dominated within a day after burn injuries. At the same time, a number of G0G1 cells also increased, along with an increase of proliferative unit and decrease of proliferation index, on account of reduction in number of G2 + M cells. We noticed a decrease in G0G1 cell population and increase of proliferation index under further development of burn injury within 3 days after registration of burns. A significant number of cells with fragmented DNA and increased parameters of proliferation unit remained on 3 and 7 day of the experiment. Also, we observed an increased S-phase index in animals with burn injuries 3 and 7 days after actual burns compared with the same index in animals with no burns.