Genetic Variations among Ten Isolates of Fusarium Equiseti (Corda) Saccardo Isolated from Fruits and Vegetables
Journal: Austin Journal of Biotechnology & Bioengineering (Vol.1, No. 5)Publication Date: 2014-10-14
Authors : Bonde SR; Gade AK; Rai MK;
Page : 1-5
Keywords : Dendrogram; Fusarium equiseti; Genetic diversity; RAPD; UPGMA;
Abstract
We studied ten- isolate of F. equiseti isolated from different fruits and vegetables, which were identified on the basis of morphological characteristics and Random amplified polymorphic DNA (RAPD) analysis of these isolates was made by using 25 different universal decamer primers to confirm the identity. UPGMA study differentiation of ten isolates into two main clusters on the basis of similarity. The aim of the present study was to assess genetic variations among ten- isolate of F. equiseti by isolated from fruits and vegetables RAPD technique. The dendrogram obtained from the data showed that hierarchical clustering separated the isolates into three groups according to their similarity coefficients. The similarity coefficients among the all isolates ranged from 0.02 to 0.15.
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