Development and Validation of UV Spectrophotometric Method for Simultaneous Estimation of Metformin and Glipizide in Tablet Dosage Form
Journal: International Journal of Applied Pharmaceutical Sciences and Research (Vol.1, No. 2)Publication Date: 2016-07-03
Authors : K. Ganesh; G. Nikitha; D. Sireesha; B. Vasudha;
Page : 56-59
Keywords : Development; Validation; UV Spectrophotometric Method; Simultaneous; Estimation; Metformin; Glipizide; Tablet Dosage Form;
Abstract
A simple, rapid and precise spectrophotometric method has been developed for simultaneous estimation of Metformin and Glipizide. The method involved estimation of Metformin and Glipizide by simultaneous equation at 272nm and 232nm respectively in their solution in water. This method was validated with respect to linearity, accuracy, precision, LOD and LOQ. Beer’s law obeyed in the concentration range of 5-25μg/ml and 20-50μg/ml for Metformin and Glipizide respectively with the correlation coefficient of above 0.99. Limit of detection and quantification values were determined to be 0.214μg/ml and 0.649μg/ml for Metformin and 0.608μg/ml and 1.854μg/ml for Glipizide respectively. Mean recovery of Metformin and Glipizide were found to be in the range of 98-102% signifies the accuracy of the method. The method was found to be precise as %RSD was less than 2.
Other Latest Articles
- Moroccan human resources between educational system and labor market
- Challenges in Implementation of Cloud Analytics
- Using ICDL Program to Enhance Students Skills on Computer Applications in Sudanese Universities
- Design and Simulation of 32 bit Floating Point FFT Processor Using VHDL
- Design MAC protocol for Cognitive Radio Wireless Sensor Networks based on network requirements
Last modified: 2016-07-05 23:22:06