Use of Polymerase Chain Reaction (PCR) Technique to Detect of Extended-spectrum β-lactamases in Klebsiellagranulomatis Isolated from Vagina of Al-Diwaniya City (Iraq) Women

ABSTRACT 100 vaginal swabs were collected from women in Al-Diwaniya city (Iraq). It had been isolated Klebsiella granulomatis from vaginal swabs then subjected all isolates under study to test the sensitivity (disc diffusion method) towards some antimicrobial and follow the PCR technique were identified extended-spectrum of genes responsible for the effectiveness of the β-lactamases antibiotics (bla TEM, bla SHV, bla CTX-M and bla AMPC) and the results showed fully resistance isolates K. granulomatis to penicillin, ampicillin and cephalosporin antibiotics (100%), while resisted to ceftazidime and cloxacillin by (88 and 80)%, respectively, compared to other antibiotics under study which showed K. granulomatis isolates different sensitivity towards it. Also identified the extended-spectrum of isolates toward the β-lactamases antibiotics by using Polymerase Chain Reaction technique which recorded the percentage of genes bla CTX-M (30/ 93.75%), bla SHV (25/ 78.12%), bla TEM (18/ 56.25%) and bla AMPC (22/ 68.15%), respectively.