Detection of Toxin producing E. Coli Isolates from Panipuri sold in Bangalore sold in Bangalore by PCR Analysis

Out of 100 samples of panipuri collected 74 were positive for E. coli. Isolates pe16, pe68, pe74, pe88 and pe93 were selected based on their protein production and multi drug resistance properties. All the five isolates were subjected for subjected to cytotoxic effect and PCR analysis. ?-haemolysis were shown by all the five isolates on micro-titre plates. PCR analysis of the isolates showed that all five are negative for Stx-1 whereas for Stx-2 only pe16 is positive and the rest four are negative.