A RP-HPLC Method Development and Validation for the Estimation of Glibenclamide in Bulk and Pharmaceutical Dosage forms

A simple, selective, linear, precise and accurate RP-HPLC method was developed and validated for rapid assay of Glibenclamide in pharmaceutical dosage form. Isocratic elution at a flow rate of 1.0 mL min -1 was employed on Inertsil-Extend C18 column at ambient temperature. The mobile phase consisted of methanol: phosphate buffer 45:55 (v/v) and the detection wavelength were at 228 nm. Linearity was observed in concentration range of 10-150 μg/mL. The retention time for Glibenclamide was 10.43 min. The method was validated as per the ICH guidelines. The proposed method can be successfully applied for the estimation of Glibenclamide in pharmaceutical dosage forms.