DEVELOPMENT OF ULTRAVOILET SPECTROSCOPIC AND THIN LAYER CHROMATOGRAPHY METHOD OF BROCCOLI EXTRACT

Broccoli contains sulforaphane, an isothiocyanate which is formed by the action of myrosinase enzyme on glucoraphanin. This sulforaphane has anticancer properties and can also help in treating many diseases like kidney disorders, skin diseases etc. The present paper highlights the development of UV and TLC method, which will be used in further research. The paper highlights the development of UV method in three media namelyDistilled water, phosphate buffer 7.4 and HCl buffer 2.3. This paper also highlights the Development of TLC method for the identification of sulforaphane in Broccoli extract. Key words: Broccoli, sulforaphane and glucoraphanin